IGF for fracture healing?

[Scott]

Anyone have research data or experience? I have recently suffered a foot fracture at my proximal 5th metatarsal. This type of fracture is notorious for poor healing due to poor anatomic circulation at this particular bone. It has no direct blood supply. I've never used peptides.

[StrappingYoungLad]

Synergistic effect of IGF-I and TGF-ß1 on fracture
healing in rats
Single versus combined application of IGF-I and TGF-ß1
Gerhard Schmidmaier, Britt Wildemann, Tobias Gäbelein, Joanna Heeger,
Frank Kandziora, Norbert P Haas and Michael Raschke
Department of Trauma and Reconstructive Surgery, Charité, Humboldt-University of Berlin, Germany
Correspondence: gerhard.schmidmaier@charite.de
Submitted 02-04-10. Accepted 02-11-16
ABSTRACT During the last few decades, knowledge
about growth factors and their function has increased.
However, little is known about the interaction of these
factors during bone growth and fracture healing. In
vitro studies have shown a higher rate of cell proliferation
and cell metabolism after the use of IGF-I and
TGF-ß1 in combination, as compared to the single use
of these factors. The purpose of this study was to investigate
a possible synergistic effect of these growth factors
in vivo, using a fracture model.
A midshaft fracture of rat tibia (n = 84) was intramedullary
stabilized with poly(D,L-lactide)-coated or
uncoated titanium K-wires. The growth factors IGF-I
and TGF-ß1, singly or in combination, were incorporated
in the coating and continuously released during
fracture healing. 28 days after fracture, we performed
mechanical tests and histomorphological analyses.
We found a greater stimulating effect of IGF-I on
fracture healing than of TGF-ß1. The combined application
of both growth factors resulted in a significantly
higher maximum load and torsional stiffness than the
use of only one of them. The histomorphometric analyses
showed an increase in remodeling of the fracture
callus in this group with less cartilaginous and more
mineralized tissue than in the other groups. Both growth
factors seem to have a synergistic effect on fracture healing
in this model.

Increasing evidence indicates that growth factors,
such as insulin-like growth factor (IGF) or transforming
growth factor-beta (TGF-ß), are important
regulators of cellular proliferation, differentiation,
extracellular matrix synthesis and mineralization
(Mohan and Baylink 1991, Linkhart et al.
1996, Trippel et al. 1996, Lind 1998). TGF-ß1
is expressed at high levels in osteoblasts during
bone development (Kim and Ballock 1993) and
healing (Joyce et al. 1990a, Steinbrech et al. 2000,
Tatsuyama et al. 2000). It affects cell proliferation
and phenotypic gene expression in vitro (Hock et
al. 1990, Centrella et al. 1991) and stimulates bone
healing in vivo (Gombotz et al. 1994, Fujimoto et
al. 1999). IGF-I stimulates the replication of osteoblasts
and the synthesis of bone matrix in vitro
(Canalis 1980, Hock et al. 1988) and healing of
bone defects in vivo (Thaller et al. 1993a).
The use of these factors individually or in
combination stimulates cell proliferation and cell
metabolism. A synergistic effect of IGF-I and
TGF-ß1 on cell proliferation, matrix synthesis
and differentiation was seen in chondrocyte cell
culture (Tsukazaki et al. 1994, Matsumura et al.
2000). Similar results have been reported concerning
the effects on proliferation and differentiation
of osteoblasts (Kasperk et al. 1990).
We studied the effects of the growth factors
IGF-I and TGF-ß1 on fracture healing when
applied locally alone or together. The tibiae of rats
were fractured in a standardized manner and the
progress of healing was evaluated radigraphically.
After 28 days, mechanical torsional testing and
histomorphometric analyses were done.


I used it when I had a bone graft on my jaw and that healed nicely.

[Scott]

SYL: what kind of dosage did you use for your jaw? Do you think it matters where I inject?

[ThaiBoxa]

I would say 50mcg is a good all-around dose. Injecting it as close to the injury as possible would provide the best benefit.

[Scott]

Update:

Worked VERY well. Used 1 vial.

Doc was impressed, xrays showing very good healing.

You'll see peptides; specifically FGF-2, being used in the future to facillitate fracture healing.

[access]

Thanks for the update!